Abrogation of Autoimmune Diabetes in Nonobese Diabetic Mice and Protection against Effector Lymphocytes by Transgenic Paracrine TGF-
نویسندگان
چکیده
Paracrine effect of transforming growth factorb 1 (TGFb 1) on autoimmune insulitis and diabetes was studied by transgenic production of the active form of porcine TGFb 1 (pTGFb 1) in pancreatic islet (islet) a cells in nonobese diabetic (NOD) mice under the control of rat glucagon promoter (RGP) (NOD-RGP-TGFb 1). None of 27 NOD-RGP-TGFb 1 mice developed diabetes by 45 wk of age, in contrast to 40 and 71% in male and female nontransgenic mice, respectively. None of the NOD-RGP-TGFb 1 mice developed diabetes after cyclophosphamide (CY) administration. Adoptive transfer of splenocytes of NOD-RGP-TGFb 1 mice to neonatal NOD mice did not transfer diabetes after CY administration. Adoptive transfer of three types of diabetogenic lymphocytes to NOD-RGP-TGFb 1 and nontransgenic mice after CY administration led to the lower incidence of diabetes in NOD-RGP-TGFb 1 mice versus that in nontransgenic mice: 29 vs. 77% for diabetogenic splenocytes, 25 vs. 75% for islet b cell–specific Th1 clone cells, and 0 vs. 50% for islet b cell–specific CD8 1 clone cells, respectively. Based on these, it is concluded that autoimmune diabetes in NOD mice is not a systemic disease and it can be completely prevented by the paracrine TGFb 1 in the islet compartment through protection against CD4 1 and CD8 1 effector lymphocytes. ( J. Clin. Invest. 1998. 102:499–506.)
منابع مشابه
Abrogation of autoimmune diabetes in nonobese diabetic mice and protection against effector lymphocytes by transgenic paracrine TGF-beta1.
Paracrine effect of transforming growth factor-beta1 (TGF-beta1) on autoimmune insulitis and diabetes was studied by transgenic production of the active form of porcine TGF-beta1 (pTGF-beta1) in pancreatic islet (islet) alpha cells in nonobese diabetic (NOD) mice under the control of rat glucagon promoter (RGP) (NOD-RGP-TGF-beta1). None of 27 NOD-RGP-TGF- beta1 mice developed diabetes by 45 wk ...
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